![]() ![]() Difference Between Template and Coding Strand Names These features, along with the sequences of the promoter, can be used by bioinformatics tools to predict genes by Ab initio method. Since the coding strand contains similar sequences to the mRNA, the unique sequences in the mRNA-like start codon, stop codon and the open reading frame can be found in the coding sequence. Therefore, bioinformatic tools like GLIMMER and GeneMark, which are involved in the finding of genes in a particular DNA sequence, depending on the coding sequence in order to predict genes in that particular DNA sequence. ![]() The coding strand contains the same nucleotide sequence of the mRNA primary transcript. The template strand in the transcription is shown in figure 1.įigure 2: Coding Strand in the Transcription RNA polymerase adds uracil as the complementary nucleotide to the mRNA strand for adenine in the template strand instead of thymine. The synthesizing mRNA is temporally attached to the template strand by forming hydrogen bonds with the complementary nucleotides in the template strand. The anti-codon is complementary to codons in the non-template or the coding strand. The template strand consists of a sequence of anti-codons which are the nucleotide triplets found in the tRNAs individually. The template strand is also called the antisense strand or the positive strand. The other DNA strand in the double-stranded DNA is called non-template. That means, the template strand is the DNA strand in the double-stranded DNA which is responsible for the amino acid sequence of the synthesized polynucleotide chain. Hence, the DNA strand, which consists of 3’ to 5’ directionality in the double-stranded DNA, may serve as the template strand in transcription. Therefore, the template strand should be directed from 3’ to 5’ in order to add complementary nucleotides to the growing mRNA strand in the 5’ to 3’ direction. Usually, RNA polymerase, which is the enzyme involved in the transcription of genes into mRNAs, adds nucleotides in the 5’ to 3’ direction to the growing strand of mRNA. The template strand is the strand which serves as the template for the mRNA synthesis during transcription. What is the difference between Template and Coding Strand The main difference between template and coding strand is that template strand only serves as the template for transcription whereas coding strand contains the exact same sequence of nucleotides in the mRNA except thymine.ģ. The template strand is responsible for the amino acid sequence in synthesizing the polypeptide chain. The other strand in double-stranded DNA, which runs from 5’ to 3’ direction is known as the coding strand. The template strand runs in 3’ to 5’ direction. During transcription, one of the two strands in the double-stranded DNA serves as the template strand. Template and coding strand are the two terms that describe the two strands in the double-stranded DNA. Main Difference – Template vs Coding Strand ![]()
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